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KMID : 1005220160310010047
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Journal of Embryo Transfer
2016 Volume.31 No. 1 p.47 ~ p.52
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The Studies on In Vivo Embryo Production using Sex-Sorted Sperm in Korean Brindle Cattle
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Jeon Hyang-A
Moon Seung-Ju
Lee Ji-Woong
Kang Man-Jong
Sohn Sea-Hwan
Kim Nam-Tae
Ko Yeoung-Gyu
Kim Sung-Woo
Kim Dong-Kyo
Seong Hwan-Hoo
Cho Young-Moo
Choi Chang-Yong
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Abstract
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The objective of this study was to investigate the result of in vivo embryo collection and pregnancy rate after embryo transfer using sex-sorted sperm of Korean brindle cattle. Donor Korean brindle cattle superovulation treated by decreasing dose of FSH injection. Embryos were recovered on 7 days after the third artificial insemination. Control group semen straw used artificial insemination contained 20 million sperm. Sex-sorted semen straws contained 4 million sperm or 10 million sperm. As for the result of the recovery of the in vivo embryos derived from sex-sorted sperm, the number of transferable embryos was significantly highly recovered to be 6.20±2.28/donor from the control group and was significantly lowly recovered to be 1.57±1.72/donor from the group treated at a sperm concentration of 10×106 (p<0.05). The number of unfertilized embryo was 0.8±1.30/donor in control group which was significantly lower than the group treated at a sperm concentration of 4×106 (p<0.05). However, there was no significant difference in the number of undeveloped ova between control and treatment groups. Pregnancy rate after embryo transfer was shown to be 35.00% in control group and 12.50% in treatment group. The karyotype analysis of the calf derived from sex-sorted sperm resulted in a similar chromosomal distribution pattern (2n=60, XX) compared to those of common Korean native cattle.
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KEYWORD
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sex-sorted sperm , superovulation , in vivo embryo , Korean brindle cattle
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